This product is a direct replacement of NEB# P7706 (Blue Prestained Protein Standard, Broad Range 11-190 kDa).
The Blue Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed.
- Allows approximate molecular weight determination when performing SDS-PAGE analysis.
- Applications include verification of Western transfer efficiency on membranes and fluorescent imaging of SDS-PAGE.
- Direct loading, additional loading buffer and heat incubation not required
- Recombinant proteins with no detectable protease contaminating activities
- Optimal stability for up to 24 months
- Product Information
The Blue Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed. The Blue Protein Standard is designed for observing protein separation during SDS-PAGE, verification of western transfer efficiency on membranes and for approximating the size of proteins (1-2). It may also be used in fluorescent imaging of SDS-PAGE.
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
P7718S | -20 |
Blue Prestained Protein Standard, Broad Range (11-250 kDa) | P7718SVIAL | -20 | 1 x 150 gel lanes |
P7718L | -20 |
Blue Prestained Protein Standard, Broad Range (11-250 kDa) | P7718SVIAL | -20 | 5 x 150 gel lanes |
Features
- Direct loading, additional loading buffer and heat incubation not required
- Applications include verification of western blot transfer efficiency on membranes and fluorescent imaging of SDS-PAGE
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.
Companion Products
- Unstained Protein Standard, Broad Range (10-200 kDa)
- Color Prestained Protein Standard, Broad Range (10-250 kDa)
Notes
- To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.
- To maximize shelf life, store at -20°C. Guaranteed stable for 2 years when properly stored.
- It is not necessary to pre-heat the unstained protein standard prior to loading on a gel.
- Recommended mini-gel load: 3 μl.
- Apparent molecular weight values for prestained proteins can be different when run on different gel types and percentages.
References
- Laemmli, UK (1970). Nature. 227, 680.
- Sambrook, J., Fitsch, EF. and Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
Protocols
- Protocol for Using the Blue Prestained Protein Standard, Broad Range for an SDS-PAGE Mini Gel (P7718)
FAQs
- How does the new Blue Prestained Protein Standard, Broad Range differ from NEB’s previous Blue Prestained Protein Standard?
- What is the protein concentration of the proteins used in the Blue Prestained Protein Standard, Broad Range?
- What volume is supplied in a small pack and a large pack of NEB’s Protein Standards?
- I notice that the molecular weights I am observing are slightly different than those reported. Can you explain?
- I accidentally left my Protein Standard out at room temperature for a number of days. Is it still okay to use?
Citations
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- P7718G_v0_10040345
- P7718L_v1_10040344
- P7718S_v1_10040343
- P7718S_v1_10060467
- P7718L_v1_10056677
- P7718G_v1_10062625
- P7718S_v1_10062624
- P7718G_v1_10065781
- P7718S_v1_10065780
- P7718L_v1_10062626
- P7718S_v1_10079387
- P7718L_v1_10065782
- P7718S_v1_10083672
- P7718L_v1_10088275
- P7718S_v1_10087442
- P7718L_v1_10094817
- P7718S_v1_10094818
- P7718S_v1_10106719
- P7718G_v1_10071059
- P7718S_v1_10108417
- P7718S_v1_10116033
- P7718L_v1_10127446
- P7718S_v1_10136938
- P7718G_v1_10116032
- P7718L_v1_10145564
- P7718S_v1_10145562
- P7718G_v1_10158153
- P7718L_v1_10155111
- P7718L_v1_10159980
- P7718L_v1_10160662
- P7718S_v1_10165204
- P7718G_v1_10167426
- P7718L_v1_10175356
- P7718L_v1_10191642
- P7718S_v1_10195945
- P7718S_v1_10211582
- P7718L_v1_10214248
- P7718G_v1_10230412
- P7718L_v1_10235837
- P7718S_v1_10238112
Safety Data Sheets
Legal And Disclaimer
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.